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unmethylated gpppg  (New England Biolabs)


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    New England Biolabs unmethylated gpppg
    Unmethylated Gpppg, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 186 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cap+analog/G(5)ppp(5)G+RNA+Cap+Struc+Analog/pm42103131-88-26-28
    Average 95 stars, based on 186 article reviews
    unmethylated gpppg - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Synthesized:

    Article Title: ACRC/GCNA is an essential protease for the repair of DNA-protein crosslinks during vertebrate development
    Article Snippet: .. RNA was synthesized using purified, linearized plasmid by performing an in vitro transcription with SP6 using the Hiscribe SP6 RNA kit (#E2070S, NEB) and a cap analog from the ARCA kit (#S1411, NEB) for improved mRNA stability. .. The resulting RNA was purified using the RNA cleanup kit (#T2040, NEB) and injected into 1-2 cell stage gcna/acrc maternal mutant embryos or into control WT embryos (1nl of the mRNA solution: 250ng/μl RNA in 0.3M KCl and 0.015% phenol red).

    Purification:

    Article Title: ACRC/GCNA is an essential protease for the repair of DNA-protein crosslinks during vertebrate development
    Article Snippet: .. RNA was synthesized using purified, linearized plasmid by performing an in vitro transcription with SP6 using the Hiscribe SP6 RNA kit (#E2070S, NEB) and a cap analog from the ARCA kit (#S1411, NEB) for improved mRNA stability. .. The resulting RNA was purified using the RNA cleanup kit (#T2040, NEB) and injected into 1-2 cell stage gcna/acrc maternal mutant embryos or into control WT embryos (1nl of the mRNA solution: 250ng/μl RNA in 0.3M KCl and 0.015% phenol red).

    Article Title: Rbm24a dictates mRNA recruitment for germ granule assembly in zebrafish.
    Article Snippet: .. After purification, the DNA templates were added to the mRNA synthesis mix containing 0.5 mM cap analog (NEB, S1407S), 0.5 mM ATP, 0.5 mM CTP, 0.5 mM UTP, 0.1 mM GTP, 20 U SP6 RNA polymerase (NEB, M0207S), 20 U RNA inhibitor (Thermo Scientific, EO0381), and 1× RNA polymerase buffer. .. The mixture was incubated at 37 °C for 1.5 h. Subsequently, the DNA templates were removed by DNase I treatment (Roche, 4716728001) at 37 °C for 15 minutes.

    Article Title: Rbm24a dictates mRNA recruitment for germ granule assembly in zebrafish
    Article Snippet: .. After purification, the DNA templates were added to the mRNA synthesis mix containing 0.5 mM cap analog (NEB, S1407S), 0.5 mM ATP, 0.5 mM CTP, 0.5 mM UTP, 0.1 mM GTP, 20 U SP6 RNA polymerase (NEB, M0207S), 20 U RNA inhibitor (Thermo Scientific, EO0381), and 1× RNA polymerase buffer. .. The mixture was incubated at 37 °C for 1.5 h. Subsequently, the DNA templates were removed by DNase I treatment (Roche, 4716728001) at 37 °C for 15 minutes.

    Plasmid Preparation:

    Article Title: ACRC/GCNA is an essential protease for the repair of DNA-protein crosslinks during vertebrate development
    Article Snippet: .. RNA was synthesized using purified, linearized plasmid by performing an in vitro transcription with SP6 using the Hiscribe SP6 RNA kit (#E2070S, NEB) and a cap analog from the ARCA kit (#S1411, NEB) for improved mRNA stability. .. The resulting RNA was purified using the RNA cleanup kit (#T2040, NEB) and injected into 1-2 cell stage gcna/acrc maternal mutant embryos or into control WT embryos (1nl of the mRNA solution: 250ng/μl RNA in 0.3M KCl and 0.015% phenol red).

    In Vitro:

    Article Title: ACRC/GCNA is an essential protease for the repair of DNA-protein crosslinks during vertebrate development
    Article Snippet: .. RNA was synthesized using purified, linearized plasmid by performing an in vitro transcription with SP6 using the Hiscribe SP6 RNA kit (#E2070S, NEB) and a cap analog from the ARCA kit (#S1411, NEB) for improved mRNA stability. .. The resulting RNA was purified using the RNA cleanup kit (#T2040, NEB) and injected into 1-2 cell stage gcna/acrc maternal mutant embryos or into control WT embryos (1nl of the mRNA solution: 250ng/μl RNA in 0.3M KCl and 0.015% phenol red).

    other:

    Article Title: Rbm24a dictates mRNA recruitment for germ granule assembly in zebrafish
    Article Snippet: Cap analog , NEB , Cat#S1407S.



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    Image Search Results


    (A) A-cap / stemloop IRES reporter RNA carrying the negative control “scramble 1” sequence was mixed into a polysome lysate made from HEK293T cells for five minutes on ice and loaded on a sucrose gradient. (B) The sucrose gradient was fractionated and cDNA was prepared from groups of fractions as shown (numbered 1 -6). qRT-PCR was performed to quantify the exogenous “scramble 1” RNA across the fractions, as compared to endogenous Gapdh mRNA. The “scramble 1” transcript migrated into polysomal fractions 4-6, even though it is not translated .

    Journal: bioRxiv

    Article Title: Evaluating the reliability of tools for mRNA annotation and IRES studies

    doi: 10.64898/2026.03.29.707813

    Figure Lengend Snippet: (A) A-cap / stemloop IRES reporter RNA carrying the negative control “scramble 1” sequence was mixed into a polysome lysate made from HEK293T cells for five minutes on ice and loaded on a sucrose gradient. (B) The sucrose gradient was fractionated and cDNA was prepared from groups of fractions as shown (numbered 1 -6). qRT-PCR was performed to quantify the exogenous “scramble 1” RNA across the fractions, as compared to endogenous Gapdh mRNA. The “scramble 1” transcript migrated into polysomal fractions 4-6, even though it is not translated .

    Article Snippet: The ARCA m 7 G-cap analog (New England Biolabs; S1411) was used at a 4:1 ratio to GFP for transcription of T7-nluc constructs, while an A-cap analog (New England Biolabs S1406), was used to generate T7HP-nluc IRES reporter RNAs.

    Techniques: Negative Control, Sequencing, Quantitative RT-PCR